A second urine sample submitted by Josh Barnett, who was to headline Affliction: Trilogy on August 1 against Fedor Emelianenko, has also tested positive for Drostanolone, the same anabolic steroid detected in his “A” sample which forced him off the card earlier this month and ultimately caused the event to be canceled and the promotion to be folded.

Josh Barnett, who was forced out of his Affliction: Trilogy headliner against Fedor Emelianenko after testing positive for steroids, has issued a statement via MySpace regarding the failed test:

“It was not a random test. I had no reason to believe there would be any issues and went in to submit my sample at the earliest possible opportunity on June 25. I never once thought there would be a problem.”

The California State Athletic Commission (CSAC) has denied a license to Mixed Martial Arts (MMA) fighter Josh Barnett after Barnett tested positive for a banned substance.

Barnett’s June 25 drug test came back positive for 2a-methyl-5a-androstan-3a-ol-17-one, an anabolic steroid. CSAC learned of the results July 21 and immediately denied a license for Barnett. CSAC also informed the promoter of Barnett’s August 1 bout against Fedor Emelianenko that the bout would not be approved.

Affliction held a conference call on Wednesday afternoon with the media to discuss Josh Barnett’s positive test for a banned substance, which has resulted in his removal from the promotion’s August 1 “Trilogy” main event against Fedor Emelianenko, and their hunt for a replacement to face Emelianenko at the pay-per-view event.

On the call, Affliction’s Tom Atencio said the promotion was in talks with 3 fighters (reported to be Brett Rogers, Vitor Belfort, and Ricardo Arona) to replace Barnett and square off with Fedor, but said nothing was official and that talks were ongoing.

A positive test for steroids will prevent Josh Barnett from fighting Fedor Emelianenko next Saturday in the main event of Affliction: Trilogy.

According to Fox Fight Game, Barnett tested positive for steroids in the first out-of-competition drug test administered by the California State Athletic Commission and will likely be issued a one year suspension.

Affliction’s Tom Atencio has confirmed to Fanhouse that, as previously reported, Josh Barnett is out of his Affliction: Trilogy headliner against Fedor Emelianenko after a pre-fight drug screening was positive for a banned substance

Atencio declined to reveal what substance Barnett tested positive for and went on to say that, although the main event is no longer intact, “at this point” the event is still expected to take place.

Josh Barnett will likely be unable to fight Fedor Emelianenko on August 1 in the main event of Affliction: Trilogy after the California State Athletic Commission reportedly failed to license Barnett for the fight for what is being called a “positive test result

According to a report from Sherdog, middleweight Vitor Belfort has agreed in principle to fight Fedor in place of Barnett, with heavyweight prospect Bobby Lashley also under consideration as a possible replacement.

On Thursday, Affliction officially announced the majority of the lineup for its August 1 Affliction: Trilogy event, which will take place at the Honda Center in Anaheim, California and air live on pay-per-view and HDNet.

Trilogy is headlined by a heavyweight matchup between top-ranked Fedor Emelianenko and Josh Barnett.

Also confirmed for the pay-per-view main card are Gegard Mousasi vs. Renato Sobral, Vitor Belfort vs. Jorge Santiago, and Paul Daley vs. Jay Hieron, as well as Paul Buentello, who is expected to take on the winner of a June 27 bout between Pedro Rizzo and Gilbert Yvel.

The complete rumored cast for TUF 10 has been revealed, Urijah Faber has been medically suspended until December 4th, the UFC is interested in Josh Barnett if he can beat Fedor, Wanderlei Silva is calling for a rematch with Rich Franklin, and much more in MMAFrenzy.com's latest Quick Hits.

On Friday, Affliction officially announced its third event, "Affliction: Trilogy", which will take place on August 1st at the Honda Center in Anaheim, California and features a main event pitting WAMMA heavyweight champion and top-ranked heavyweight Fedor Emelianenko against challenger and former UFC champion Josh Barnett.

Top-ranked heavyweight Fedor Emelianenko and contender Josh Barnett have agreed to meet at Affliction's third event on August 1st at the Honda Center in Anaheim, California, according to MMA Fanhouse. Though bout agreements have not yet been signed, the bout is expected to be finalized and announced in the coming days.

M-1 Global has announced that heavyweights Gilbert Yvel and Josh Barnett will fight at Affliction: Day of Reckoning on January 24th in Anaheim, California:
M-1 Global is pleased to announce that Gilbert Yvel, one of its top heavyweight competitors, has agreed to terms on a three-fight contract with Affliction Entertainment.
Yvel will make his Affliction debut against [...]

Josh Barnett (25-5) may finally have an opponent for his scheduled bout at Affliction: Day of Reckoning on January 24th at the Honda Center in Anaheim, California.
According to Five Ounces of Pain, former EliteXC heavyweight Brett Rogers (8-0) is Affliction’s preferred opponent for Barnett, though the matchup has not yet been officially signed.
Barnett, a top-10 [...]

World Victory Road today announced an updated lineup for Sengoku 6, which is scheduled to take place on November 1st at the Saitama Super Arena in Saitama, Japan.
Heavyweight Josh Barnett headlines Sengoku 6 against an opponent to-be-announced, with the event also slated to feature the conclusion of the promotion’s lightweight and middleweight tournaments.
Added to the [...]

Fedor Emelianenko, the world’s near-consensus top heavyweight and among the planet’s best pound-for-pound fighters, will not be competing on New Year’s Eve and will also not meet Josh Barnett early next year at Affliction’s second event.
Affliction Vice President Tom Atencio recently alerted MMA Junkie of the status of the promotion’s top fighter.
“I’ve been told actually [...]